Comparative Investigations of the Phase Shift Distribution by
Interferometric Phase Microscopy
and Fs-Laser-Pulses Analysis on Biological Cells

J. Beuthan¹, O. Minet¹, . Vajda², and G. Müller^{1, 3}

¹ Institut für Medizinische Physik und Lasermedizin, Freie Universität, Berlin, Germany

e-mail: j.beuthan@lmtb.de, minet@zedat.fu-berlin.de

² Institut für Experimentalphysik, Freie Universität, Berlin, Germany

³ Laser- und Medizin-Technologie GmbH, Freie Universität, Berlin, Germany

Received July 12, 2002

Abstract—The first investigations on the metabolism of yeast cells in vivo by means of the retrieval of the
phase distribution from femtosecond cross correlation measurements show a characteristic distribution of the
phase shift of coherent light. The obtained phase shift distribution is compared with the phase shift distribution
retrieved from a series of phase sensitive measurements carried out on a set of optically identically oriented
samples by means of quantitative phase-microscopy. First comparisons show better statistic results by means
of the fs-laser-pulse analysis at a considerably reduced measuring time. Consequently, this method is better
suited to high throughput investigations.

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